The adequacy of sedation and analgesia were assessed using physical examination and vital signs taking at 30-min intervals by experienced animal care technicians.
The effects of the UCMS and fluoxetine in both diet conditions were assessed using physical (coat state and body weight) and behavioural tests (the reward maze test and the splash test).
Changes in headache disability were assessed with HIT-6 (29, 30) and quality of life was assessed using physical (PCS) and mental (MCS) component summary scores of SF-36v2 (31).
HRQoL was assessed using Physical and Mental Component Summary (PCS, MCS) scores from the Medical Outcomes Study 12-Item Short Form Survey Instrument (SF-12v2), a multipurpose, generic HRQoL instrument [ 25].
These health-related (i.e., strength, flexibility) and skill-related (i.e., balance, coordination, speed) components of physical fitness [ 13] were assessed using physical fitness tests (i.e., Bourban TMS test, standing long jump test, 20-m sprint test, stand-and-reach test, jumping sideways test, Emery balance test, Y balance test).
Self-reported difficulty with physical function will be assessed using the physical function subscale of the Likert version of the WOMAC [ 25].
Physical activity will be assessed using the New Zealand Physical Activity Questionnaire – short form (NZPAQ-SF), a modified version of the International Physical Activity Questionnaire IPAQQ) [ 64, 65].
Physical activity will be assessed using (11) the Rapid Assessment of Physical Activity [ 34].
At age 13, girls' enjoyment of physical activity was assessed using the Physical Activity Enjoyment Scale and their daily minutes of moderate-to-vigorous physical activity (MVPA) were assessed using objective monitoring.
Physical activity was assessed using the Physical Activity Score for the Elderly (PASE) [ 23].
