To confirm the identity of these cell populations, gene lists generated by comparison of neighbouring cell populations using linear model analysis for significantly differentially expressed genes (P<0.046) (supplementary material Table S1) were assessed for expression of 80 genes known to be non-ubiquitously expressed along the neural axis.
The 107 highly expressed unique tags in the normal libraries were assessed for expression changes in CIN III.
Monocyte populations were assessed for expression of CD14 and CD16 using flow cytometry, and data was expressed as percentages of CD45 positive cells (i.e. all leukocytes).
Six different human promoters (FLT-1, VEGF, Cox2, INOS, DR5 and survivin) were assessed for expression of mRNA in D54 MG cells 2 h after exposure to 2 Gy radiation using quantitative RT-PCR.
Following transfection, after 60 72 h depending on the cell line, cell lysates were assessed for expression levels of PTEN using western blot analysis as per standard protocols.
Ten control and twenty passage treatment fish were assessed for expression of SBDPs using biotinylated anti-mammalian αII-spectrin (Fig. 3A).
Following transfection, after 60 to 72 h depending on the cell line, cell lysates were assessed for expression levels of different proteins using western blot analysis as per standard protocol.
Supernatants harvested from cultures described in "Proliferation assays" were assessed for expression of 27 cytokines using the Bio-Plex human cytokine 27-plex panel (Bio-Rad) according to manufacturer's recommended protocol.
A selection of 26 transcripts of interest dispersed along the intergenic regions was assessed for expression changes in the presence of various stress conditions including pH, temperature, oxidative shocks and growth in a stringent medium.
