A 56-year (1947 2003) time series was assembled to detect long-term changes in the overall size of seagrass meadows around Baraulu and Nusa Hope (Figs. 3, 4).
Sequences were assembled to 105 contigs (4,414 bp long on average).
Sequences were assembled to 147 contigs (4,402 bp long on average), using GS De Novo Assembler version 2.0 (Roche).
The other two amplicons, E5 - E6 (10,231 bp) and E7 - E8 (3,353 bp), were assembled to form chromosome II, 12,675 bp long; these two amplicons overlap by 347 bp in rrnL and 470 bp in NCRII-3.
Sequences were assembled to the average of 5,123-bp-long 824 contigs for the CW-CMS genome, and 4,966-bp-long 4,966-bp-long 4,966-bp-longS genome, using the GS De Novo Assembler Software (Roche).
Paired-end sequence reads were assembled to reconstitute the ca. 281-bp-long amplicons.
Since most metagenomic reads cannot be assembled into long contigs, specialized statistical gene prediction tools have been developed for short and anonymous DNA fragments, e.g. MetaGeneAnnotator and Orphelia.
The Gigantor project has a bit more of an official page than the Gundam, where you can see that they're already well underway in creating the enormous pieces to be assembled into the chubby, long-nosed bot.
A broader outgroup selection and more comprehensive gene sampling (e.g. including nuclear genes) may resolve the root of Osmundaceae more reliably in the future, providing a molecular data set can be assembled that is immune to outgroup long-branch attraction.
As a PTD event, by definition, must involve at least a single full exon, we expect that such an event will usually be too long to be assembled as a full-length chimeric transcript.
