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Once replication is over and the replication checkpoint is turned off, HR centers can be assembled to complete the repair of the accumulated ssDNA gaps and broken ends.
In Escherichia coli, the structure-specific nuclease RuvC serves both of these purposes by processing HR repair intermediates and by mediating RF cleavage so that a new RF may be assembled to complete the replication process.
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These sequences were assembled to complete sequences with SeqMan II™ (DNASTAR inc).. Positions where the nucleotide could not be determined with certainty were coded with the appropriate IUPAC code.
Sequencing reactions of the target fragments were performed in both directions by 'primer walking' using a Big Dye Terminator Cycle-Sequencing Kit (Applied Biosystems), and overlapping fragments were assembled to complete the sequence of the entire genome.
The multiple segments obtained by sequencing with different primers were assembled to complete sequences using S EQM AN II™ (DNASTAR®).
However, none of the ESTs from C. salei, N. antipodiana, Aphonopelma sp. or P. tepidariorum could be assembled to a complete coding sequence and therefore these sequences were not included in our analyses.
In addition, the short reads cannot be assembled to give complete sequence of complex plant genomes [ 21] and repeat sequences cannot be aligned unambiguously with the reference genome [ 22].
In addition, these two robust assemblies could be assembled to create a more complete non-redundant consensus reference L. loa genome, using the methods established for the generating the non-redundant consensus human genome.
We show that short reads can be assembled to a near-complete chromosome level for a eukaryotic species lacking prior genetic information.
Current sequencing of other clones indicates that the Ectocarpus genome contains several other genes of EsV-1 that could be assembled to form an apparently complete viral genome.
Individual genetic alterations and their contribution to different disease stages and specific manifestations of SS might one day be assembled to depict a more complete and integrated picture of SS.
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