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In the present study, some cysteine residues were introduced at the N-terminus of calmodulin by genetic engineering, and the resulting engineered calmodulin could be assembled efficiently with oriented manner.
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(55) Catenane (interlocking ring-like structures) was assembled efficiently without enzymatic ligation.
The second protein that the reviewer alludes to (the RD-EspP chimera) is assembled efficiently in vivo.
This tethering allows the microtubules to be assembled more efficiently.
This will provide researchers with valuable insight for designing more complex higher-order structures that can be assembled more efficiently.
For example, a combination of 11 could most efficiently be assembled in 5 rounds of cloning (Figure 6a).
The results showed that gene-containing regions could efficiently and accurately be assembled into contigs, even at read coverages as low as x10.
CPMV cannot be assembled in vitro, nor can virus-like particles be efficiently produced in heterologous systems.
As a next step, we analyzed how efficiently multiple level 1 modules could be assembled into a level 2 construct.
In such cultures, mouse PGCs were able to efficiently form primary oocytes capable to be assembled into primordial or primary follicles.
Long reads are optimal for initial genome and transcriptome characterisation because longer pieces are assembled more efficiently than shorter pieces [ 25].
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