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Working with the genome of Rhodopseudomonas palustris, Schadt et al. [ 35] demonstrated that long reads generated from PacBio sequencing can help resolve assembly difficulties in a repetitive region spanning 1.5 kb, which otherwise cannot be assembled by using Illumina sequencing.
The test rig is modelled as a single-degree-of-freedom system and must be assembled by using stiff bearings and torsionally compliant shafts.
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More than 725,000 trimmed reads were assembled by using de novo assembly and analyzed according to BLAST searches (Technical Appendix Figure 1 and Table 1) (6, 7 ).
Raw sequence data were trimmed to remove any primer-derived and low-quality sequence; gene sequences were assembled by using a viral assembly tool (www.jcvi.org/cms/research/software).jcvi.org/cms/research/software
More than 234,000 trimmed reads were assembled by using de novo assembly in CLC Genomics Workbench 4.5.1 (CLC Bio, Aarhus, Denmark) and analyzed according to nucleotide and translated nucleotide BLAST searches (http://blast.ncbi.nlm.nih.gov/Blast.cgi).nih.gov/Blast.cgi
Pryosequencing reads representing 130 Mb of data (ca. 20.4× coverage) were assembled by using the Newbler assembler version 1.1.03.24 (Roche) into 1276 contigs with an N50 contig size of 8455 bp.
The shotgun sequences were assembled by using the GS de novo assembler.
The paired end reads were assembled by using a de novo genome assembly program Velvet version 0.7.03 (19 ), and a multicontig draft genome was generated for each sample.
The clean reads of each gene detected in RNA-seq sequencing analysis was assembled by using Trinity software (de novo assembly) [ 40] and the assemble results were provided in supplemental files.
The sequence reads were assembled by using the Edena de novo short-reads assembler (Genomic Research Laboratory, Geneva, Switzerland).
The on-farm composting system was assembled by using currently available tools in common farms.
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