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To purify PCR fragments by ultrafiltration, the Centricon-100 column should be assembled as directed.
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We used Overlap Extension PCR cloning [22] or restriction enzymes and ligase, to create recombinant plasmids; BioBricks were assembled as directed in the BioBrick Assembly Manual (NEB, Ipswich, MA).
Wild-type genomes were assembled, as follows.
Histone octamers were assembled as follows [31].
TMA blocks were assembled as follows.
The collection was assembled as follows.
Can they be assembled, as Rams hoped, without a manual?
8 12 The Lisbon Cohort of MSM was assembled as a facility-based open prospective cohort in a community-based voluntary HIV counselling and testing service directed at MSM.
The explants were assembled as described above.
Data were assembled as longitudinal survey panel data for analysis.
Gonad and Intestine images were assembled as previously described [87].
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