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All samples for all analytes will be assayed in duplicate and the analytical variance (SDA) will be calculated from the differences between the duplicate measurements.
Serum CRP, IL-6, TNF and the other cytokines listed above will be assayed in duplicate with ELISA (CRP high-sensitivity kit; R&D Systems, Oxford, UK) or enhanced cytokine bead array flex kits (Becton Dickinson, Franklin Lakes, NJ, USA) using the manufacturer's reagents and standards.
Similar(58)
All samples were assayed in duplicate.
All samples were assayed in duplicate at ambient temperature (21 25 °C).
All lung homogenate test samples were assayed in duplicate.
A 10-fold dilution series of a control plasmid was assayed in duplicate and spanned 103 to 109 copies.
All test sera were assayed in duplicate.
Serum samples were assayed in duplicate.
Samples were assayed in duplicate in 2 independent runs.
The neutralization activity of each VHH was assayed in duplicate.
Serum from each animal (50 µl) was assayed in duplicate.
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