This will be ascertained using a simple question within the follow-up measure.
Outcomes measured at the level of families and children's centres will be ascertained using a range of tools delivered as summarised in Table 1 and described below.
Whether a RHR-lowering strategy results in a benefit in renal and/or cardiovascular outcomes cannot be ascertained using a cohort follow-up and requires a clinical trial to translate from epidemiology to clinical care.
Factors with possible impact on outcome differences between and within networks will be ascertained using a variety of tools, such as surveys, recurring questionnaires, and in specific instances, site visits to explore details if permitted.
The CHD cases were ascertained using an annual follow-up questionnaire and validated by an End Point Committee through a review of the medical records.
Microsatellite alleles were ascertained using an ABI Prism™ 310 automatic sequencer (Applied Biosystems, Foster City, CA, USA).
APP was ascertained using an algorithm comprising of two steps, as illustrated by Fig. 1.
Life events were ascertained using an 11 item questionnaire (derived from [ 29]).
Unit costs for both procedures were ascertained using an 'ingredients' (bottom-up) approach from the healthcare perspective, with the costs of individual components added to calculate a unit cost for each circumcision.
Two neurophysiological parameters are ascertained using an EEG amplifier (19 electrodes, 10 20 system): a) Quantitative EEG (QEEG) is recorded during 6 minutes resting condition (3 minutes eyes closed and 3 minutes eyes open) and during the first, last and follow-up training session, with the same equipment used for feedback but at 19 electrodes.
