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However, the power of UHTS can be applied to sequence shorter DNA regions obtained by sequence capture or conventional PCR in multiple patients, i.e. to a procedure that is more similar to current routine setups in medical genetic laboratories.
Due to this a very strict threshold could be applied to sequence coverage (95%) during the motif search.
However, it remained to be demonstrated whether the RF methodology could be applied to sequence feature selection for siRNA classification.
Here, we propose a single framework which estimates the similarity of small protein fragments and can be applied to sequence, structure or both simultaneously.
Exactly the same process can be applied to sequence by using distributions of amino acid probabilities at each of the k sites within a class.
To demonstrate that BigFoot can be applied to sequence data from vertebrates, we analyzed a 256 base pair region previously identified as the major regulatory element of human α-globin (αMRE) [ 40].
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Móricz's [1] and Gaposhkin [2] showed that some properties of sequences of independent random variables can be applied to sequences consisting of independent blocks.
The method cannot be applied to sequences where such information is either unavailable or insufficiently available.
The program, however, can only be applied to sequencing data produced with an Illumina Genome Analyzer.
In addition to the ability to sequence DNA, massively parallel sequencing can be applied to sequencing RNA [ 22].
Hence, before it can be applied to sequencing data, ClinSeK scans the reference genome to obtain information of paralogous sites that share sequence similarity with the target sites.
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