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This could be applied to miRNA genes.
Our assay could easily be applied to miRNA target identification by substituting shRNA expression with miRNA expression.
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The remarkable ability of CaP-HDz-NCs to deliver RNAs is not confined to siRNA delivery but can also be applied to miRNA-based RNAi.
To overcome these issues, several modifications, some of which already developed for siRNAs, can be applied to miRNAs.
A similar statistical analysis to determine differentially expressed mRNAs was applied to miRNA expression data to identify differentially expressed miRNAs.
The same approach was applied to miRNA data, where 3197%7%) of 32 clusters identified in the Chinese dataset were validated in the Caucasian dataset (Fig. 1D).
Several computational approaches have been applied to miRNA gene prediction using methods based on sequence conservation and/or structural similarity [ 3- 7].
The above methods were applied to miRNA and mRNA expression dataset in 237 tumor tissue samples and 10 normal tissue samples with 1,697 differentially expressed mRNAs and 149 differentially expressed miRNAs.
Methods developed for microarray normalization (e.g., lowess or quantile normalization) have also been applied to miRNA-seq data [ 63].
Most of these methods have been applied to miRNAs, the class of ncRNAs that currently holds the highest potential for clinical applications.
In this method, the novel technology which enabled multiplexed direct digital counting of RNA molecules [ 28] were applied to miRNAs with some modification.
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