Precision micro-replication process was applied to duplicate HML on a flexible polyethylene terphthalate (PET) substrate by UV-forming process.
A pooled quality control sample prepared in the same manner was applied to duplicate spots on each proteinchip used in the experiment as a reproducibility control.
Depending on the software application, filters were applied to duplicates, ESTs, probes with only family similarity and hypothetical proteins.
Our method can be applied to pairs of duplicates and requires that, for each pair, we can distinguish between parent and child copies and identify their single-copy ortholog (referred to as "outgroup gene" here, and as "ancestral gene" in [ 10]) in a closely related sister species.
The same tricks that are used to teleport your character around or duplicate gold pieces could be applied to real-world systems to steal data or gain access to financial transactions, depending on what the architecture is set up to do.
Furthermore, methods using sequence coverage to identify repeats (e.g. Malde et al., 2006; Phillippy et al., 2008) should not be applied to pyrosequencing data without first filtering duplicates.
The SAM tools package [ 30] was used to convert mapping results to BAM format, and then the Picard program (http://picard.sourceforge.net) was applied to eliminate duplicated reads generated during the process of library construction.
Bland-Altman analysis was applied to 81 duplicate samples from 22 patients undergoing CPB or percutaneous coronary interventions (PCI), covering the full clinical range of ACT values.
The same process was applied to a duplicate chip experiment, showing essentially the same cell response to toxic insult (data not shown), in an attempt to estimate the variability of IC50 measures.
The formula of the expression distance (E) between gene 1 and 2 is as follows: (2) E ^ 12 = ∑ k = 1 m (x 1 k − x ¯ 1 ) − (x 2 k − x ¯ 2 ) 2 / (m − 1 ) The method of Gu et al. [ 45] was applied to identify duplicate genes.
