Interestingly, when a similar technique was applied to discovery of cancer antigens, numerous out of frame peptides were discovered (Lin et al., 2007, Wang et al., 2005).
This system has been used with a variety of bacteria, and, in particular, has been applied to discovery of virulence genes in several microbial pathogens.
It is the systematic analysis of low-molecular-weight metabolites in biological samples and has become an important tool in clinical research and the diagnosis of human disease and has been applied to discovery and identification of the perturbed pathways.
A SNP validation rate of 96.8% indicated that the AGSNP pipeline is a high-throughput SNP discovery tool that can be applied to SNP discovery in two or more genotypes from low to high complexity genomes.
The methods may also be applied to the discovery and design of inhibitors for use as antibiotics or as modulators of NO signaling.
There is no reason other than the usual obstacles to innovation that the principles couldn't be applied to drug discovery in general.
Any animal model of disease capable of predicting therapeutic outcomes in the human patient is a valuable tool that can be applied to the discovery of new therapeutics.
Metabonomics can potentially be applied to the discovery of tumor metabolic pathways, the investigation of metabolic responses to treatments [20], and the identification of tumor biomarkers of these responses [11], [12], [15].
Similarly, the same procedure can be applied to the discovery of compounds that bind to other amyloid proteins, for use as either toxicity inhibitors or imaging agents for amyloid diagnosis.
This review surveys reported substrate phosphorylation site inhibitors and methods that can be applied to the discovery of such inhibitors, including a discussion of the challenges inherent to these screening methods.
