The plates were washed and the samples were applied in appropriate dilutions in 0.5% BSA/PBS.
Primary antibodies were applied in appropriate blocking solution overnight at 4°C.
Secondary antibodies were applied in appropriate blocking solution: Horseradish peroxidase-conjugated-conjugated anti-rabbit/anti-mouse (Dako, Glostrup, Denmark) at 1 2000 and 1 3000, respectively, for 1 h at room temperature.
In additional to conventional cytogenetic analysis, fluorescent in situ hybridization (FISH) was applied in appropriate bone marrow specimens using 13q14.3, 13q24, and 17p13.1 (p53) deletion probes and 14q32 (IGH) break apart probe (Vysis, Des Plaines, IL, USA) according to the manufacturer's instructions.
After washing, the following antibodies were applied in appropriate dilutions in PBS buffer with 2.5% bovine serum albumin and 0.05% Tween-20 for 4 hours at RT, or overnight (ON) at 4°C: BC-3 cell-supernatants diluted 1 100, or F-78-POD at a final concentration of 2 μg/ml respectively.
Natural gas power generation technologies should be applied in an appropriate place and act as an important back-up technology for intermittent generation technologies.
Finally, the developed functionals are independent of the coordinate system and can be applied in any appropriate system of coordinates chosen for convenience.
Moreover, this prominent tolerance opens up more opportunities for a recombinant host to be applied in an appropriate fermentation process, using either growing cells or high-density resting cells, with different types of expression and process controls, e.g. batch, fed-batch, continuous or multi-stage continuous (Garcia et al. 2011).
Such biomarkers must also be applied in the appropriate clinical trial context.
Such specificity shows that larvae have a memory specific to the quality of punishment, a memory that can specifically be applied in the appropriate situation.
