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These results suggest that the peptide-HA matrices are useful as 2D and 3D matrices and can be applied for tissue engineering as a synthetic basement membrane.
Emerging technologies, for example, piezosurgery, optical coherence tomography, or microendoscopy, may be applied for tissue ablation [ 20, 21].
With careful implementation, the quantitative apparent diffusion coefficient derived shows good measurement reproducibility, which can be applied for tissue characterization, the assessment of tumour response and disease prognostication.
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Scaffold-free cell sheet-based constructs have been applied for tissue repair due to their high rates of nutrient diffusion, abundant deposition of ECM, and interactions with cell membrane proteins, including growth factors, ion channels, and cell-to-cell junction proteins.
Such techniques are even being applied for tissues where scaffolds have shown success (e.g., skin, bone, and cartilage) [ 18].
This cryopreservation technique could be applied for hard tissue reconstruction to ease the cell preparation method prior to time of use.
These results demonstrate that cryopreservation has no effect on the phenotype, proliferation or osteogenic differentiation of human ASCs, showing cryopreserved human ASCs might be applied for bone tissue engineering.
The results demonstrate that cryopreservation has no effect on the cell phenotype, GFP expression or osteogenic differentiation of UCB-MSCs, showing that cryopreserved GFP-labeled UCB-MSCs might be applied for bone tissue engineering.
Thus, CS/nHAp-modified decellularized goat-lung scaffold can be applied for bone tissue regeneration.
It also signifies that the cells maintained their osteoblastic phenotype within the CS/nHAp-modified decellularized goat-lung scaffold, which can be applied for bone tissue regeneration.
Using our spot-selection method we provide a series of tissue specific gene sets which can be applied, for example, to study tissue-specific factors in different diseases.
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