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HRM can be applied for mutation detection and SNP genotyping in medical research [ 21].
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The same method is applied for mutation rate, with average mutation rate for a cluster being substituted for average gene maximum multiplicity rate.
Therefore, the authors proposed that this system could be applied for insertional mutation of multiple gene in Clostridium [30].
The same explanation can be applied for the mutation rate dependency of the U-parasite evolution (however, the bistability was not observed).
This method corrects for deleterious mutations, as the relative abundance of deleterious mutations is reduced at higher frequencies, but cannot be applied for beneficial mutations which have increased relative abundance at higher frequencies.
Although we describe this recombination mapping method for the third chromosome, the principle of using pairs of dominant markers can be applied for mapping mutations on other chromosomes.
Standard preimplantation genetic diagnosis (PGD) cannot be applied for de-novo mutations (DNM), because neither origin nor relevant haplotypes are available for testing in single cells.
It is promising that the designed biosensors can be applied for detection of prostate cancer mutation in the future.
The authors state that FunSeq will be most effective in the analysis of tumour genomes, but can also be applied for the analysis of germ line mutations [ 22].
As DASE is a sensitive functional index for pathogenic mutations, it can be applied for validating these VUS at a genome-wide scale as well.
The described primer/probe design strategy can also be applied for the detection of other polymorphisms or disease causing mutations.
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