After a thorough dialysis the solution protein was collected and stored at 4°C. 12.5% SDS-PAGE was applied for confirmation of the expressed product.
When a normal distribution of measurements was not predicted, the Wilcoxon rank sum test (Mann-Whitney U test) was applied for confirmation using the JMP software (version 7.0.1; SAS Institute, Cary, NC, USA).
Additionally, M-PCR should be applied for the diagnosis or confirmation of BV, which would lead to an earlier diagnosis to prevent possible complications in specific women, without the impediments of high cost, long assay times, and difficulties in workflow.
Research on using these tools and techniques for Health Informatics is critical, because this domain requires a great deal of testing and confirmation before new techniques can be applied for making real world decisions across all levels.
Q5 high-fidelity DNA polymerase was used for all PCR reactions to minimize the introduction of possible error bases, and Sanger sequencing was applied for fidelity confirmation.
The polymorphic SSR markers were applied for subsequent confirmation in all F1 lines.
Direct infusion and isocratic (acetonitrile/water/acetic acid, 50 50 0.1, v/v; all other LC parameters are specified above in the previous section) RPLC MS scan and MS/MS product ion scan analysis was applied for the confirmation of identity and for assessment of purity of the labeled DNPHs.
Two gold standards were applied for the analysis as it is known that microbiological confirmation detects only approximately half of all pediatric TB cases when applied optimally and will therefore overestimate sensitivity and underestimate specificity.
The procedure was applied for the update of the Goesgen seismic PRA and for the confirmation of a revised safe shutdown earthquake for the Goesgen nuclear power plant.
Since variances of recoveries were not homogenous for β-IO and DHA over the tested period, Welch test was additionally applied for confirmation.
