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Since the pathway maps are primarily produced as a tool for communication it is critical that they are easily understandable and the notation can be applied and read by biologists with minimal training.
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To stop the reaction, 100 μl 2M HCl was applied and plates were read at 450 nm in a Polar Star Omega plate reader (BMG Labtech).
After washing of the wells, Eu3+-conjugated streptavidin was applied and the signals were read by time-resolved fluorometry.
Glycerol-PBS mounting medium was added to each well, a coverslip was applied, and the slides were read at a magnification of 400x with an epifluorescence UV microscope.
If the signature genotyping groups had not formed after the initial amplification, additional amplification cycles (usually 5-10) were applied, and the samples were read again.
Alkaline phosphatase yellow liquid substrate (Sigma, # A3469) was applied, and the OD was read at 405 nm with a reference filter at 490 nm.
Application of the aforementioned MaSuRCA filtering procedures for jumping libraries was applied and yielded ∼4.5 million distinct mapped read pairs for downstream overlap layout consensus assembly.
Thus, a fragmentation step is applied and as a consequence the reading direction, traditionally indicated by poly-A tail primers, is lost.
This can be applied to read out, for example, two FLIM FRET measurements of FRET biosensors or intermolecular FRET reporting protein protein interactions.
Control signal pulses can be applied to selectively read the state of a data cell and to selectively set a data cell to a desired data state.
Now its technology will be applied to reading the profiles, messagees, comments, and statuses of Tagged users.
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