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This SSP depletion-replenishment strategy outlined here would be applicable to expression of recombinant proteins in industrial crops that generally have large repertoires of endogenous SSP genes.
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It is worth noting that this approach is applicable to expression data obtained through deep sequencing (potentially improving sensitivity for low abundance genes and cross-hybridization problems of current microarray technology).
We demonstrated that this technique is applicable to expression analyses of tissues placed under different environmental conditions and collected at different time points of organ development, as well as genetic mutants and transgenic plants as exemplified in Table 1.
Both of these technical developments will be applicable to building gene expression atlases of additional genetic perturbations in the future.
Thus, this vector should be applicable to most protein expression in yeast Saccharomyces cerevisiae and it is available for other researchers on request.
Unlike GBNet that relies on gene clusters generated from multiple microarray experiments, UMMI can be applicable to a single gene expression experiment.
Therapeutic approaches based on repression of cyclin D gene have also been investigated (Tiedemann et al, 2008; Dong et al, 2010) and may be applicable to EOCs presenting aberrant CCND2 expression due to DNA-copy number gains.
These QC criteria should be applicable to minimize experimental variation in gene expression microarray experiments.
The reversible male sterility system developed in Arabidopsis by manipulating MYB80 expression should be applicable to all major crops.
However, this technique should be applicable to any organism for which gene expression data can be generated and is thus of potential interest for a variety of applications in metabolic and cellular engineering.
It should also be noted that while control of CD25 expression may be applicable to naive CD4+ T cells and the Th1 differentiation pathway, other stages of T cell responses (e.g., memory response) and other differentiation pathways (e.g., Th17 and Tfh) are likely to be counter-regulated by IL-6 and Tregs through a different control point.
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