A pyrenyl derivative, located at the DEA block, allowed motion of this site to be monitored via TRAMS in aqueous solution: a significant reduction in the mobility of this label was apparent at concentrations in excess of the critical micelle concentration, CMC, of the diblock copolymer.
Similarly, binding of substrate to the peripheral anionic site of AChE, responsible for its characteristic allosteric inhibition, was apparent at concentrations higher than 2.5 mM regardless of the source of the enzyme.
Minimal apoptotic cell death was apparent at concentrations up to 40 pg/ml (P>0.05 versus control) but apoptotic cell death was observed at concentrations of 60 pg/ml and above with a significant (P<0.01 versus control) increase to 24% Annexin V-positive cells.
However, inhibition of yeast was found to be apparent at their concentrations exceeding 100 mM and lower concentrations than 100 mM gave higher ethanol yields than fermentations with no aliphatic acids included [ 46].
We therefore reasoned that any inhibition of endogenous wild-type IDH1 or IDH2 by transgenic IDH1 or IDH2 mutants would be apparent at this substrate concentration.
We cannot exclude that apoptosis might be apparent at later time points or with higher drug concentration (data not shown).
Moreover, significant developmental effects are apparent at blood concentrations as low as 4 μg/dl (Ballew et al. 1999).
Labeled bands at 50 and 90 kDa were apparent at higher concentrations, but they failed to saturate and are likely nonspecific adducts.
DU145 cells were fundamentally very resistant to copper with no significant inhibition of growth being apparent at metal concentrations of 100 μM and lower (Fig. 1A).
We found little or no effect of leptin on matrix biosynthesis, proteoglycan breakdown, or nitric oxide production in vitro, whereas the proinflammatory effects of leptin are apparent at superphysiologic concentrations [ 48, 49].
