A total of 51 genes, out of 65 (78%), were annotated in cluster 2/6.
Surprisingly, we obtained 69,393 unique sequences, and of which 51,448 could be annotated, in total 48,725 genes, including 14,531 clusters and 34,194 singletons.
Videos will be annotated in order to derive the information.
The tandem repeat of MSHORT1 elements could account for all 65 pre-miRNA genes that are annotated in the cluster.
Among the predicted CDSs, 2,918 (80.9 %) genes were predicted in the Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) database, 2,483 (68.9 %) genes were annotated in the Cluster of Orthologous Groups of proteins (COG) categories, while 3,056 (84.8 %), 1,418 (39.3%%) and 3,006 (83.4 %) genes were applicable within nonredundant (NR), SwissProt and TrEMBL databases, respectively.
The longest M region (28,934 nt) corresponds to a region containing a Ty (yeast retrotransposon) and the rDNA clusters (only one cluster is annotated in the reference genome) on chromosome XII, whereas many M regions exhibit much shorter lengths with a minimal length of 50 nt, limited by the read length resolution.
Nine of 101 probesets in cluster 8 are annotated in GO as participating in the inflammatory response, which is 8.2 times more than expected by chance (p = 2.2 × 10-6).
The major proteins in each cluster were annotated in carbohydrate metabolism, stress, defense, cell wall metabolism, protein process, and other metabolic processes.
Transcript clusters were annotated in R using the Bioconductor package hugene10sttranscriptcluster.db (Affymetrix Human Gene 1.0-ST Array Transcriptcluster Revision 8 annotation data, assembled using data from public repositories).
A total of 22 defensin pseudogenes were annotated in the major mouse defensin gene cluster region on mouse Chromosome 8 (Additional File 1: Supplemental Table S2), while ten pseudogenes were annotated in the corresponding human defensin cluster.
