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574 gel spots were significantly identified by mass spectrometry and among these, 285 distinct proteins expressed in the naïve midgut could be annotated for function and/or localization.
Short reads need to be assembled into contigs, which are similar in length to a gene, so that they may be annotated for function inference.
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Genes were annotated for function as described [ 27].
The SNVs were annotated for function using ANNOVAR [ 15], and only non-synonymous mutations were retained.
This work has made available approximately 3000 sequences from pre-smolt salmon, one third of which have been annotated for function, biological process and cellular component.
The resulting TAs (or contigs), along with the unassembled singleton ESTs, are annotated for function through searches against a protein database and loaded into the Plant Pathogen Transcript Assemblies database.
The phages in S. aureus are a remarkable source of untapped genetic diversity [ 45] with more than 60% of predicted protein-encoded ORFs that cannot be annotated for structure or function [ 46].
The most expressed genes from the transcriptome (first 1% of mean normalized expression) regardless of phenotype were annotated for biological function in BLAST2GO [ 35] and enrichment analysis resulted in upregulated expression of 134 slimmed GO-terms in biological function.
By sequence similarity searching for known proteins, 27,962 (57.16 %) unigenes were annotated for their function.
In a first step, all genes were annotated for protein function using InterProScan [ 79].
Transcripts significantly affected by ACTH treatment were annotated for their function according to Gene Ontology database [ 33].
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CEO of Professional Science Editing for Scientists @ prosciediting.com