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In this way, both large, gene rich scaffolds (e.g. CM3.5.1_scaffold00036, 38, and 50) which accounted for most of the newly anchored sequence, as well as many smaller scaffolds less than 1 Mb in size could be anchored and oriented.
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Over 83% of the final assembly was anchored and oriented using four publicly available de novo linkage maps.
Based on the published linkage map [ 17], scaffolds were anchored and oriented into 18 linkage groups.
For example, BAC/PAC draft sequences or contigs were anchored and oriented on the rice genetic maps – these contigs were further augmented by BAC-end sequencing (via Sequence-Tagged Connectors) and contig-end walking.
The fourth is the sequence-ready BAC/PAC physical map, and these BAC/PACs were anchored and oriented on the genetic map which forged a minimum tiling path of BAC/PAC clones for each of the 12 rice chromosomes – it comprises 3,466 BAC/PAC clones showing 46 gaps [ 8]. * Ch. = Chromosome; est.
Although a number of links to the currently existing genetic, cytogenetic, and RH maps were possible, a multitude of contigs and scaffolds of the available bovine genome sequence resources still have to be anchored and/or oriented on the chromosomes.
Scaffolds with only one SNP marker could be anchored but not oriented because of the lack of markers.
The best result of the five-parameter set was determined by the greatest number of anchored and oriented scaffolds.
The genome of the melon (Cucumis melo L). double-haploid line DHL92 was recently sequenced, with 87.5 and 80.8% of the scaffold assembly anchored and oriented to the 12 linkage groups, respectively.
In addition, there are only two marsupials species with a gene map of sufficient density or an anchored and oriented genome assembly.
The main purpose of constructing the map was to anchor and orient scaffolds onto the pseudochromosomes for the B. oleracea sequencing project.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com