Following normalization to U6 (miRNA) or RPLPO (mRNA), the data were exported and analyzed in RealTime Statminer software, as suggested by the manufacturer.
In addition to EGFR IHC, EGFR mRNA expression was analyzed by realtime PCR in 130 of the 357 patients.
Target gene expression in presence or absence of the inhibitors was analyzed by realtime PCR (Additional file 3, Table S2 and, for a schematic overview, Addditional file 4, Figure S2).
Transfection of siRNAs against Mcl-1 downregulated Mcl-1 mRNA and protein levels as was analyzed by quantitative realtime PCR (q-PCR) and immunoblot analysis (Fig. 2A,B).
Transcript levels of DEFA 1/3 and 4 were analyzed by quantitative realtime PCR and compared with healthy salivary gland tissue.
Differential gene expression was analyzed by realtime-PCR with the iCycler® Thermal Cycler (Bio-Rad, Munich, Germany).
An additional cohort was analyzed using aCGH, realtime-PCR, and immunohistochemistry.
Samples were analyzed with a Geniom Realtime Analyzer (GRTA, febit GmbH, Heidelberg, Germany) using the Geniom Biochip miRNA homo sapiens.
Samples were analyzed using a Geniom Realtime Analyzer (GRTA, CBC Comprehensive Biomarker Center, Heidelberg, Germany) with the Geniom Biochip miRNA Homo sapiens.
Samples were analyzed with the Geniom Realtime Analyzer (GRTA, febit gmbh, Heidelberg, Germany) using the Geniom Biochip miRNA homo sapiens.
