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In the research methodology, the thermal comfort variables would be analyzed by quantitative methods on the time series.
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Shoots were analyzed by quantitative RT-PCR.
The mRNA levels of IL28 (B) and IL29 (C) were analyzed by quantitative real-time PCR.
(A) Relative amounts of SRS5 mRNA in various organs were analyzed by quantitative RT-PCR.
After 48 h, the mRNA level of GAPDH was analyzed by quantitative PCR.
The level of USP33 mRNA was analyzed by quantitative RT-PCR.
b ABA-regulated expression of OsPYLs was analyzed by quantitative RT-PCR.
Livers were analyzed by quantitative reverse transcriptase polymerase chain reaction for lipid metabolism-related gene expression.
Proteins were analyzed by quantitative western blots of cerebellar homogenates prepared from male or female neonates.
In order to get a clue about the function of CsPI, mRNA accumulation was analyzed by quantitative real-time PCR.
The resulting cDNA was analyzed by quantitative real time PCR (qPCR) with EcoTM Real-Time PCR System (Illumina, Gene Company Limited) (China, Hongkong).
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