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Cell-wall polysaccharides can be analysed using fluorescence microscopy after labelling with a green fluorescent protein tagged carbohydrate-binding molecule specific for cellulose (Lacayo et al. [2010]).
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HER2 gene copy number was analysed using fluorescence in situ hybridisation (FISH).
BHK cells and 40 μm sliced-oocytes were analysed using fluorescence microscopy.
The EGFR gene copy number was analysed using fluorescence in situ hybridisation (FISH).
The formation of actin stress fibres as an index of endothelial cytoskeletal reorganisation was analysed using fluorescence microscopy.
Frozen sections of HCE were analysed using fluorescence microscopy for the evaluation of apoptosis (terminal deoxynucleotidyl transferase mediated dUTP nick end labelling), inflammation (ICAM-1) and proliferation (Ki67).
The EGFR and HER2 gene copy number were analysed using fluorescence in situ hybridisation (FISH) on 5 μm thick paraffin sections.
For DNA sequencing, plasmid DNAs prepared with a commercial column (Qiagen) served as templates in cycle sequencing reactions performed using dideoxy chain termination (Sanger et al, 1977) Sequence were analysed using fluorescence DNA sequencers.
Fluorescence was analysed using a fluorescence microscope (BX51; Olympus, Tokyo, Japan).
24 h after transfection the living cells were analysed using a fluorescence microscope (DM IRB, Leica Microsystems, Wetzlar, Germany) equipped with a 100-W Hg light source (HO 103W/2, Osram, Munich, Germany) and a standard FITC filterset.
The slides were analysed using a fluorescence microscope.
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