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However, these six items were kept in the questionnaire to be analysed separately in future studies.
Owing to the sequential design of the study, the qualitative and quantitative components will be analysed separately in a sequential qualitative-quantitative analysis.
Each of the somatic conditions will also be analysed separately in order to investigate whether different conditions generate different levels of distress.
Both components of the composite will also be analysed separately, in accordance with regulatory guidance, and these additional analyses will be considered as part of the primary endpoint and not as secondary outcomes.
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To determine whether gender differences in effect exist, men and women were analysed separately in multivariate analyses.
However, since every patient is analysed separately in N-of-1 trials, the outcome measures do not have to be the same for all patients in a series.
Hence, the data were grouped into these three (normally distributed) classes and each class was analysed separately in all statistical tests.
When the anaemic patients were analysed separately in the Cox regression model, age over 75 years and ACS at presentation were identified as independent predictors of MACCE at 12 months, and chronic renal impairment, age over 75 years and ACS at presentation as independent predictors of all-cause mortality.
Since the risk of relapse and death is the highest during the first 5 years, particularly for ER-negative disease [ 27, 41], two periods of time were analysed separately in this study: the first 5 years after diagnosis and the subsequent years.
Based on the relationships that could be determined with a degree of confidence, the sequences were divided into two clusters to be analysed separately (as shown in figure 1).
We only considered autosomal chromosomes (chr1 22) to avoid the additional complication of gender (in which case, male and female samples would need to be analysed separately).
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