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Compound 2 was shown to be an efficient substrate for PPAT whereas 5 was a very poor DPCK substrate.
RSH turned out be an efficient substrate for PHB production from a recently isolated Bacillus cereus PS 10, and yielded higher PHB amount than that obtained with glucose (8.6 g/L in glucose based medium vs 10.61 g/L in RSH based medium) after response surface methodology (RSM) based optimization.
Our idea is that overexpression of cellulase in a living plant may lead to partial degradation and loosening of cell wall, and such cell wall can be an efficient substrate of cell wall-degrading enzymes produced by microorganisms by enhancing accessibility of the enzymes to the substrates during the saccharification process.
Thus, BMAL1 appears to be an efficient substrate of GSK3β.
More recently the nucleotide product of AZT excision, AZTp4A, was also specifically shown to be an efficient substrate for RT [45].
The "floxed" allele of Bcl-x, Bcl-xfl/fl, in which exons 1 and 2 are flanked by loxP sites, has been shown to be an efficient substrate for the Cre recombinase, producing a null allele following recombination in other tissues [23] [25].
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A free hydroxy group at carbon 3 is not a prerequisite, however, since 1-O-hexadecyl-2-desoxy-2-amino-sn-glycerol was an efficient substrate with an apparent Km value of 3.8 μM (versus 15.7 μM for sphingenine).
H2A.Z exchange therefore promotes specific patterns of histone modification and reorganization of the chromatin architecture, leading to the assembly of a chromatin template that is an efficient substrate for the DSB repair machinery.
Cyclocreatine, an analog of creatine, is an efficient substrate for creatine kinase, but its phosphorylated form is a poor phosphate donor in comparison with creatine phosphate.
Hence, a protein composed of C-terminus 87 amino acids of acc is an efficient substrate for Biotin Protein ligase [8].
We then tested the single-stranded (SS) M13 template and figure 1A shows that it is an efficient substrate for dCMP incorporation using axolotl LSE (lanes 7 to 9).
More suggestions(15)
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