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The gene encoding aldolase can be amplified using the standard polymerase chain reaction (PCR) from a genomic DNA preparation using the Qiagen kit and primers flanking the gene with engineered restriction sites.
All the modules shown in Figure 3 can be amplified using the same forward primer, but they require different reverse primers (Table 1).
One strain of HRV-C (N1) could not be amplified using the P1 and VP2 extreme primers and was amplified using primers in 5'UTR and VP4/VP2, respectively (Table 1).
As expected, PCR-based assays showed that many molecular markers for PRRSV could be amplified using the primers specific to the unique gene fragments (e.g., ORF6 3'-NCR) of PRRSV (Fig. 4B).
The presence of the intI1 gene on a plasmid was determined in seven of the E. coli isolates (D18, D22, D79, D87, D111, D398 and D318) where cassette arrays could not be amplified using the standard primer pair L1 and R1.
Current sequencing technologies often require DNA samples to be amplified using the polymerase chain reaction (PCR).
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Responses were amplified using the Axoclamp-2A amplifier (Molecular Devices, Inc).
The target DNA was amplified using the species-specific primer sets (Table 1).
Hybridization probes were amplified using the primers hLZ-S and hLZ-A (Table 1).
Both cel5 and cel48 genes were amplified, using the PCR conditions described above.
The denitrifying functional genes were amplified using the primer pairs shown in Table 1.
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