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In an ideal situation, completely conserved primer binding sites could be targeted so DNA from all prey targeted would be amplified simultaneously, with equal efficiency.
All loci could be amplified simultaneously with the Qiagen® Multiplex PCR Kit (Qiagen, Venlo, The Netherlands).
Due to the high sequence similarity among Alu elements, thousands of Alu elements could be amplified simultaneously with one primer set.
Optimal properties of an STRP include: high heterozygosity, strong and specific PCR amplification, capability to be amplified simultaneously with other STRPs (multiplexed), sharp bands on gels, easy and accurate scoring of allele sizes, relatively low mutation rate, and appropriate position along the genetic map.
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gDNA and all MDA DNA were amplified simultaneously in duplicate, and genotypes were determined.
Artificially-degraded DNA, artificially-degraded MDA DNA and genomic DNA were amplified simultaneously in duplicate for each SNP analyzed.
The glutathione S-transferase (GST) GSTM1 and GSTT1 fragments were amplified simultaneously as proposed by Chen et al., 1996 [ 31], using β-globin as positive control.
For QRT-PCR assays, a single external RNA control can be added to the RNA sample and amplified simultaneously with the target sequence (two-color system) or independently in a replicate well (one-color system).
DNA was amplified prior to detection with NALFIA strips.
PCR products were amplified using GS3-PstI and then digested with PstI at 37 °C.
DH5[alpha ] Escherichi coli-competent cells were transformed with prkDTL5F11; DNA was amplified, isolated, and sequenced.
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