To determine whether the ratio of utilization of two AUG codons is altered by binding of an antisense LNA oligonucleotide to various locations in a 5' leader other than that of AUG1, we generated a series of mRNA constructs with isosequential 5' leaders by inserting an LNA-target sequence into different parts of the 5' leader of the (CAA)16 CAT-FLAG mRNA (Figure 5A).
FP insertion into the membrane was altered by binding to TMD.
Access to the pocket is apparently controlled by the rotameric state of residue F103, which is altered by binding of the long-chain blockers.
If the mutations occur at codons 12, 13, 59, 61, 63, 116, 117, 119 or 146, its structure is altered by binding sites for guanine, affecting normal function.
This property of mHDx-1 could conceivably be altered by iAb binding, leading to variable transcription rates of HDx-1 in the presence of the various iAbs.
In addition, the binding sites of many TFs are so similar that they do not allow a reliable prediction of function; clearly, sequence preferences can be altered by the binding context [23].
This supra-fold dynamics can, in principle, be altered by ligand binding to protein domains, which can change both individual protein domain structures as well as their relative arrangement [3], [4].
Accessibility of the myristoyl moiety may be altered by ligand binding [ 5], changes in pH [ 6] phosphorylation [ 7], or proteolysis [ 8], reversing membrane localization.
In fact, the efficacy of hERG blockers might be altered by protein binding of the drug (Webster et al, 2001), or perhaps progressive degradation during long incubation.
Since daycare, school, office, and service hours usually cannot be altered by individual workers, they constitute a binding schedule constraint.
