For comparison, the Eucalyptus leaves were also extracted with Soxhlet extraction, using two different solvents, and hydro-distillation methods.
Leaf samples were also extracted with 1% (w/v) HCl in methanol, and the anthocyanin contents were assayed spectrophotometrically.
The water phase was also extracted with ethyl acetate, washed with sodium bicarbonate, dried over magnesium sulfate and concentrated under reduced pressure (Additional file 1: Figs. S3, S4).
As with the flagellar complex, the purified flagellum was also extracted with 1% NP-40 and 1 M KCl, which further removed membrane and soluble components.
Non-inoculated YPD was also extracted with ethyl acetate and concentrated by evaporation in the same conditions.
Data on coexisting cardiovascular conditions in each patient were also extracted with the use of all relevant ICD codes.
The whole nucleotide sequences of the transposable elements, if possible, were also extracted with the assistance of UniPro uGENE software.
Features were also extracted with regard to the frequency domain: the peak frequency, spectral centroid and bandwidth were computed as characterization indexes for the extracted from the TCD signals [ 45].
The propolis used in this study was Chinese red propolis from Shandong, China, and was also extracted with 95% ethanol at room temperature for 24 h, and then filtered to obtain its ethanolic extract.
We also noted that a small amount of matrilin-3 was also extracted with buffer 1 in Comp DelD469 and Comp T585M cartilages compared to wild type (Fig. 2A, insert).
