In a mathematical model, a start culture of 1000 cells having a random replication rate, according to a Poisson distribution an average replication rate of 0.5 hr−1, was allowed to replicate (and age).
The longer Ebola is allowed to replicate in humans, the greater the risk that it will become more contagious.
With the aim of determining the optimal mutation rates that promote adaptation to S2, each population was allowed to replicate under a range of values of the new error rate (μ2) between 0.001 and 0.05.
P-gp DKO MEF monolayers were grown on 10-well glass slides (Thermo Fisher, Portsmouth, NH), infected with T. gondii at an MOI of 3 and parasites were allowed to replicate for 30 h at 37°C. 10 µM GF120918 was added in the last 4 h of replication, then cells were fixed with 3.6% formaldehyde, blocked and stained with anti-T.
Virus-containing supernatant was removed, and virus was allowed to replicate for 4 h.
Also, as they are administered live, the organism is allowed to replicate within the host allowing in vivo gene expression, thus making them less expensive [ 5].
The mutated genes were then re-introduced into the virus, and the mutant viruses were allowed to replicate in cells for several generations.
When repair is complete, nucleosomes are re-established, but the pattern is distinct from that seen prior to the break or when the repaired cells are allowed to replicate.
Molecular analysis of the specific kinds of mutations induced when a plasmid containing BPDE residues was allowed to replicate in human cells showed that BPDE induces mainly base substitution mutations, predominantly G C to T A transversions.
