Sentence examples for be alkylated during sample from inspiring English sources

By contrast, palmitoylated cysteines are left with exposed thiol groups after ABE, and will be alkylated during sample processing by iodoacetamide treatment prior to gel electrophoresis.

Samples were alkylated in-gel; first samples were reduced by addition of 75 μl of 10 mM DTT in 0.1 M NH4HCO3 for 45 min at 65°C.

Subsequently, samples were alkylated with 30 mM iodoacetamide (IAA) in the dark for 30 minutes at room temperature.

Samples were alkylated with iodoacetamide.

Reduced samples were alkylated using iodoacetamide (5.5 mM) (Sigma-Aldrich).

The reduced samples were alkylated and digested by trypsin (Sigma, St . Louis MO, USA) overnight at 37 °C.

After pooling, samples were alkylated with 10 mM dithiothreitol (DTT) and 50 mM iodoacetamide (IAA).

Samples were alkylated in gel with iodoacetamide (Sigma) and digested overnight with trypsin gold (V5280, Promega).

Finally, samples were alkylated with a final concentration of 5 mM idoacetamide (IAA; Sigma-Aldrich).

Most of the gastric tumour DNA samples were alkylated (5/6; 0.087 +/- 0.097), whereas the DNA of the associated mucosa was alkylated less frequently (2/7) and to a lesser extent; (0.017 +/- 0.030; P = 0.07).

Subsequently, the DTT solutions were removed and samples were alkylated with 50 mM 2-chloroacetamide in ABC, for 20 min, at room temperature, in the dark.