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Each aligned read was assigned a mapping quality that indicates whether the read has a unique alignment or can be aligned to multiple positions in the genome [ 22].
Using an informatics pipeline that allowed up to two mismatches in a read to map to the reference, we found that 43.9% of the B73 fragment reads had a single best alignment to the reference, 46.7% could be aligned to multiple positions, and 9.3% could not be aligned to the reference at all.
One exon sequence is possible to be aligned to multiple regions in the unannotated genome.
A wild goat scaffold could be aligned to multiple domestic goat loci or chromosomes.
If reads can be aligned to multiple locations in the genome, their exact placement is ambiguous and assigned a map quality score of zero (MQ = 0).
First, a relatively short read naturally has more chances to be aligned to multiple sites of the reference genome and thus to be filtered out as an "ambiguously mapped read".
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Exons that were aligned to multiple genomic locations of their own reference genome at current cutoff were filtered out.
Exons that were not aligned to the same genomic location as WGA annotation or were aligned to multiple genomic locations using current cutoff were removed.
The aligned reads were post filtered so that the reads with mapping quality 0 that are aligned to multiple places in the genome were removed.
After the alignment to the cassava genome [ 41], 1,185,928 tags (54.6%) were aligned to unique positions while 229,629 tags (10.6%) were aligned to multiple positions and the remaining 757,678 tags (34.9%) could not be aligned.
In simulating the RNA cis-splicing mechanism, a cDNA is thought to be generated by trans-splicing when it is aligned to multiple non-contiguous genomic loci and the fusion junction obeys canonical GU-AG splice site.
More suggestions(15)
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