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Research can be aligned by using a common definition, thereby avoiding misinterpretations.
The protein-coding genes of the individual orthologous groups can be aligned by using MUSCLE or MAFFT.
Two simple POGs (and DAGs in general, [ 14]) can be aligned by using an extension of the Smith-Waterman algorithm.
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Total sequences were aligned by using the MAFFT aligner (25 ) implemented in Geneious version 5.5 (26 ).
Full-envelope protein sequences for each serotype were aligned by using the multiple alignment tool of MEGA5 (www.megasoftware.net).net
All sequences were aligned by using the multiple sequence alignment editor ALIGN-IR (LI-COR), and consensus sequences for every gene sequenced were determined.
The gltA sequences of 22 Rickettsia species were aligned by using the multiple-sequence alignment program ClustalW supported by the Infobiogen website (http://www.infobiogen.fr).fr
Sequences were aligned by using CLUSTAL W [ 127] with default multiple alignment parameters.
Sequences for each Nme family were aligned by using MUSCLE [ 86] with default multiple alignment parameters and identity matrix were obtained with BioEdit 7.0.9 software.
The sequences were aligned by using the phylogeny tree program [ 15], and the alignment was corrected manually.
Sequences were aligned by using the PRANK (Löytynoja and Goldman 2008).
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