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These estimates of hypothetical changes of gene expression were used solely for purpose of prioritizing predicted targets and narrowing down list of predictions in order to select those that are most likely to be affected in experimental groups of animals and would be more reliable candidates for validation experiments.
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We were able to uncover several functional pathways including metal homeostasis that were affected in our experimental situations.
The analysis software tool GeneTrail [ 10] can be used for comparative analysis of transcriptome data to identify functional clusters or pathways rather than single genes that are affected in one experimental condition compared to another.
We first determined whether the integrity of the BBB was affected in our experimental model of seizure by measuring EB dye extravasation into the brain parenchyma at 2 and 4 h after KA injection in WT mice (n = 10), at 2 h in Nsp −/− mice (n = 11), and at 4 h in tPA −/− mice (n = 7) (Fig. 2A).
Although the syntactical organization of the song was affected in our group of experimental starlings, the phonological structure of song units did not differ from those produced by wild-caught birds (see Fig. 2), suggesting that NCM might be involved in categorizing songs, not only depending on their acoustic structure, but also depending on their correct use in syntactical context.
It also enables rapid optimization of video tracking settings to improve quantification of automated parameters, and suggests that spatiotemporal organization of zebrafish swimming activity can be affected by various experimental manipulations in a manner predicted by their anxiolytic or anxiogenic nature.
However, the amount of procaspase-1 was less affected in experimental endotoxemia probably showing that conversion of procaspase-1 to caspase-1 was affected.
In addition, lipid metabolism was often affected in experimental animals.
The blood-testis barrier (BTB) does not change morphologically and the integrity of the BTB is not affected in experimental cryptorchidism (EC) [5], [6].
Expression of core clock genes was found to be affected early on in an experimental mouse model of osteoarthritis, further supporting a role of clock genes in the initiation and progression of osteoarthritis [ 9].
The results in the bilateral insula may also be affected by the presence of identical pairs in our experimental paradigm.
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