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However, despite the multiple proteins and pathways known to be affected by cell adhesion molecules in general and ICAM-2 in particular, complete membrane-to actin linkages that regulate CAM function in tumor cells have been difficult to identify.
Subcellular distribution of LRP6-ICD does not appear to be affected by cell type as we showed it is primarily cytosolic in CHO, HEK 293T and COS (data not shown) cells which suggests LRP6-ICD functions primarily as a positive modulator within the Wnt pathway.
DNA methylation is tissue specific, and it could be affected by cell type.
For both experiments, cells were maintained in serum-free medium during the course of assays to avoid any augmented migratory behavior that could be affected by cell proliferation.
Some previous studies have also shown that tumor FDG uptake could be affected by cell differentiation, proliferative rate potential, microvessel density, and hypoxia, which are all consistent with the biological behavior of malignant cells[ 23 25].
Lastly, since HIF-1α protein expression can also be affected by cell density/confluency [ 42, 43], protein expression in LTLTCa and MCF-7Ca cells at both approximately 50% and 95% confluencies were also analyzed.
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The assembly and disruption of such complexes is yet another level of regulation of signalling, which in vivo might be affected by cell-to-cell contacts.
Silencing by shRNAs interrupted by a group I intron could be used to monitor self-splicing activity in cultured mammalian cells, and the efficiency of self-splicing appears to be affected by cell-type specific factors, demonstrating the potential effectiveness of a trans-effector.
We further describe specific TGFβ-induced intracellular signaling cascades that are affected by cell- and tissue-level mechanics.
Speed graphs were done also using a phone app which may or may not have been affected by cell reception at the time.
Both molecular and mechanical signaling between cells in a growing tissue are affected by cell division.
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