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Galectins are not glycosylated [35] and therefore their production and function may not be affected by blocking alpha-1,2-mannosidase.
In HPL1D cells, Intgerin αV, MMP2 and TGFBIP regulation by TGF-β is affected by blocking the p38 MAP kinase pathway, none of the gene regulations by TGF-β seem to be affected by blocking the ERK pathway and the induction of TMEPA by TGF-β is dependent on the JNK pathway (Fig. 6).
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While we cannot distinguish between these possibilities at the present time, the latter explanation seems likely to us since we and others have found using microarray analysis of HDI treated cells, that hundreds of cellular transcripts induced by viral infection are affected by blocking histone deacetylase activity [23], [42].
In addition, we could investigate whether extravascular metastasis of HCC is affected by blocking PVTT formation.
The expression of ORF28 is affected by blocking DNA replication [ 27] showing ORF28 is a secondary or tertiary gene.
It was found that the induction of TSP-1, which is a TGF-β regulated gene only in A549 cells, is affected by blocking the integrin-linked signalling pathway.
Furthermore, we recently showed that the expression of two KNOXI genes (HBK2 and HBK4) was affected by blocked PAT during embryo development in P. abies (Larsson et al., 2012).
Furthermore, our data strongly indicates that off-target effects do not only derive from additionally inhibited kinases in line with the fact that within the human genome over 2000 other nucleotide-dependent enzymes can be found [35] which potentially may be affected by NCEs blocking an ATP binding site.
Employers in several sectors said they were concerned that key staff would be affected by a block on EU workers staying in the UK.
This technology can also be affected by obstructions; an object blocking an infra-red sensor's view of one or more infra-red light sources can result in the system obtaining incorrect positional information.
We further tested whether phagocytosis of ACs by human monocyte-derived GC-treated macrophages could be affected by pre-treatment with blocking antibodies against either β3 integrin or β5 integrin.
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