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Whereas it would be advantageous to sequence all available isolates within a lineage, this approach is still prohibitively expensive and requires SNP detection by whole genome comparisons.
Although it can sometimes be useful to enrich for a target sequence when studying larger, eurkaryotic genomes where coverage is otherwise limiting [31], [32], in the case of HIV, it would be advantageous to sequence the entire genome to gain insights into the composition of complex viral populations and investigate the dynamics of their evolution.
It may be advantageous to sequence more samples using WES, and gain statistical power, than to sequence more of the genome.
It may thus be advantageous to sequence from the 5′ direction if there is a need to maximise the number of detected microsatellite sequences when developing microsatellite markers for C. japonica.
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Selecting a suitable amplicon for mutation detection is a pre-requisite for establishing efficient and successful screens, and several considerations need to be taken into account: 1) In the large majority of cases, it will be advantageous to include as much coding sequence as possible and avoid intron or intragenic sequence.
On a preliminary basis, it will be advantageous to calculate a guiding parameter, net sequence length, or the actual length reduced by the number of degenerated sites.
The difference between the y component of the Z curves for C. muridarum and E. coli chromosome sequences may be advantageous to understand the appearance of the separate base usages in C. muridarum.
Owing to alternative splicing patterns, however, it can be advantageous to design siRNAs against the common sequences that occur on all spliced and unspliced HIV-1 transcripts, including the early spliced viral mRNAs that contain tat, rev, and nef reading frames.
We reasoned that when the desired end product of a ChIP experiment is a sequencing library, it would be advantageous to perform library reactions during the ChIP step rather than after DNA purification.
It would be advantageous to have a method that is applicable directly to short sequences, of the kind extracted from samples in modern metagenomic research.
It would be advantageous to display RNA nanoparticles of diverse shape and size derived from a given primary sequence.
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