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Open reading frames attributed to heavy metal resistance were additionally validated by sequence comparison with NCBI-NR using BLAST program.

Where minimum value imputation analysis was performed, our results were additionally validated by a stability analysis imputing changing fractions of the minimum values obtained for each response, which confirmed our main analysis.

Cases in Germany were additionally validated by diagnostic records.

Our findings were additionally validated by Sanger sequencing of the respective genomic loci.

The annotation of the zebrafish best hits was additionally validated by comparisons to the ZFIN database (Table 4).

The expression of proteins corresponding to several of the above defined functional gene clusters was additionally validated by immunohistochemistry on tissue microarrays.

The above observation is additionally validated by faster viral clearance in the upper respiratory tract and a significant decrease in MDA levels in MDA+0/V birds after vaccination.

Requirement of Cl− current in release of DNA was additionally validated by the cells in either Na+-free or Cl−-free medium, and subsequently DNA release was completely inhibited in Cl−-free medium.

Finally, robustness of our strategy was additionally validated by the demonstration of NRGN, a low-abundant molecule previously thought to be brain specific, in human glomerular endothelium in vitro and in vivo for the first time.

Expression of BCR/ABL was additionally validated by immunohistochemistry. Muscles from immunized mice with the BCR/ABL-pIRES-hIL-2 (g) and BCR/ABL-pIRES (h) showed a predominant staining pattern while the empty plasmid control did not (f).

Mad2 and Kid1 depletion had been previously validated in our laboratory (Meraldi et al., 2004; Wandke et al., 2012), Sas-6 depletion was validated by counting centrioles in all experiments; Mad2 and Sas-6 co-depletion was additionally validated by immunoblotting; KIF2a/MCAK depletion in Sas-6-depleted cells was validated by immunoblotting.

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