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Survival data (e.g., institutionalization, mortality) will be additionally analyzed using Cox regression.

Survival data will be additionally analyzed using Kaplan-Meier survival curves and the log-rank test.

For GSE5460, raw expression values obtained from Affymetrix GENECHIP software were additionally analyzed using DNA-Chip analyzer (dChip) custom software (www.dchip.org).

The conceptual amino acid sequences of these proteins were additionally analyzed using three algorithms (MitoProt, Psort II, and TargetP) which calculate the N-terminal region of a given protein that could support a mitochondrial leader sequence.

In order to avoid pseudogene amplification, Pou5f1 expression was additionally analyzed using the primer Mm00658129_gH Pou5f1 TaqMan gene expression assay (Applied Biosystems) and the TaqMan Fast Universal PCR Master Mix Applied Biosystemss), following manufacturer's instructions.

The relationship between performance and connectivity was additionally analyzed using 2-tailed Pearson correlations.

Longitudinal data from in vivo μCT were additionally analyzed using generalized estimating equations (GEEs; IBM).

EGFR mRNA levels were additionally analyzed using TaqMan mRNA assay according to the manufacturer's instructions (Applied Biosystems).

A number of samples were additionally analyzed using HRGC/low-resolution maspectrometrytry with negative chemical ionization mode and DB 5 (15 m, 0.25 mm i.d., 0.1-μm film) column.

Alignments were collapsed to contain only unique sequence types/alleles in COLLAPSE 1.2 [ 74] for phylogeny estimation, and intron sequences were additionally analyzed using the full number of detected alleles.

To compensate for the uncertainty caused by missing values from drop-out individuals, data were additionally analyzed using the multiple imputation technique with 10 replacements at random in order [ 21] based on the confirmation that the missing pattern was completely at random [ 22]; these results are presented in brackets.