Sentence examples for be added to cells from inspiring English sources

For cell culture experiments CM was used undiluted (SFRP3 100%) or diluted 1∶2 with culture medium (SFRP3 50%), and additionated with growth factors prior to be added to cells.

GFP-, RFP-, or luciferase-expressing P. berghei sporozoites were dissected from salivary glands of infected female Anopheles stephensi mosquitoes into DMEM medium prior to be added to cells or injected into mice for in vitro and in vivo infections.

DCF diacetate (DCFDA) will be added to cells of interest for 30 min. DCFDA is prepared in ethanol and diluted into myocyte culture medium to a final concentration of 5 mM.

Supporting this notion is our observation that far more ebselen needs to be added to cells to inhibit HCV replication than is needed to inhibit HCV helicase in vitro and the fact that Chockalingam et al. reported a similarly modest effect of ebselen on HCV RNA replication.

Unlike many cell death assays, this method uses only two dyes that can be added to cells together without a washing step, requires minimal handling or optimization, and is easily analyzed using multivariate methods available in multiple commercial and open-source software packages to enable quantification of single cells.

Cryoprotective agents (CPA) should be added to cell suspension before freezing and removed after thawing.

EdU (Thermo Fisher Scientific) was added to cells 1 h before fixation.

After incubation, samples were added to cells and residual luciferase expression was measured after 24 h.

BFA-bodipy (250 ng/ml) was added to cells to demonstrate rapid accumulation of toxins in Golgi and ER.

Caspase-3/7 Green Ready reagent was added to cells (2 drops/mL) and allowed 30 min incubation at 37 °C.

5 µM 2-NBDG was added to cells in low glucose medium (1 g/L D-Glucose) for 30 min.