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Briefly, serial dilutions of the test solutions were performed to allow 0.02 1000 ng of each sample to be added in triplicate to wells of 96-well plates coated with 50 ng human epidermal growth factor receptor (hEGFR).
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Each dilution from 1 10 to 1 1012 was added in triplicate.
Following this incubation, the mixture was added in triplicate to a 96-well black-sided plate (100 μL/well).
To this, 0.1 ml of inoculum (containing 10 CFU of A. acidoterrestris cells) was added in triplicate.
The mixture of insulin and the anti-insulin antibody was added in triplicate to the insulin-coated wells, and the plate was incubated for 1 h.
100 μl samples of each CD4 T cell culture were added in triplicate to a 96-well plate and incubated for 6 h with 1 μCi per well of [3H] thymidine.
Drugs were added in triplicate at timepoints of interest.
Briefly, cytokines were added in triplicate at decreasing concentrations.
Compounds were added in triplicate and the cells were incubated for three days.
Briefly, 100 µL of bacterial suspensions (100 bacteria/cell) were added in triplicate to the wells coated with HT29 cells.
Irradiated immature and CD40L-stimulated LC were added in triplicate in graded doses to 2×105 purified allogeneic T cells in 96-well round-bottom plates.
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