After a washing step, a 1 10,000 diluted HRP-goat anti-rabbit IgG secondary antibody and HRP-goat anti-chicken IgG secondary antibody was added accordingly and incubated at 37 °C for 0.5 h.
Specific concentrated homogenization buffer was added accordingly to maintain the correct buffer condition for each test.
An additional score of 1 is added accordingly for the presence (not severity) of each of the following secondary parameters: hypertrophy (enlarged cells), inflammation, oedema, eosinophilic granular cells, circulatory damage (e.g. haemorrhage, telangiectesias (dilated blood vessels), congestion, and the presence of bacterial (e.g. epitheliocystis, Tenacibaculum spp).
Water was added accordingly to obtain a total volume of 50 μl.
AE Buffer was added accordingly to the PCR premix to reach the final volume of 30 μl.
In each plate, TNP-specific standard IgG1, IgM (BD Biosciences, Franklin Lakes, NJ), and IgG3 (a gift from Dr. Thomas Waldschmidt, University of Iowa, Iowa City, IA) were added accordingly.
New items were added accordingly and a number of items were rewritten in the remaining scales so that items reflected the underlying construct more closely or to simplify the language.
These circled incomplete/unattended items are already numbered in priority (smallest number to largest) and should be added and prioritized accordingly to the next days' OPA list.
Link l pi will be added to H t accordingly.
Accordingly, R can be added into the CHOP therapy in one of three ways: as an induction only (RCHOP) as both an induction and a maintenance therapy (RCHOP-R) or not at all (CHOP).
