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These can be adapted for single trees using additional tree covariates like competition indices (Pretzsch 2009).

The utilization of next generation sequencing (NGS) approaches has the capability of identifying all expressed sequences, achieving massive dynamic ranges, having resolution down to the single nucleotide level [ 11– 13], and has been adapted for single cell transcription studies [ 1– 3].

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Similarly, PCR-based approaches can also be adapted for targeted single-cell mutation detection when the important driver mutations are known (95).

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The model is adapted for the single link failure scenarios.

Different methods for DNA isolation based on heat lysis were tested, that is, heat lysis in water and heat lysis in a commercial product for DNA purification were adapted for a single colony from previous reports [ 5, 18, 20, 23] (Table 1: DNA isolation).

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Traditional qPCR methods were adapted for use with single cells to accurately quantify the fold differences between BCR-ABL and ABL expression levels down to less than single-cell quantities, as defined by typical quality control descriptions (such as linearity of the standard curve for titrated RNA, Figure S-1, Supporting Information).

Later, this system was adapted for use in CHO cells for single miRNAs, which yielded relatively low levels of over-expression for various mature miRNAs, ranging from 1.2 to 2.3-fold [ 32], depending on the overall expression level.