We focused on the processes of cell culture using microcarrier systems which can be adapted for large-scale production and serum-free medium to avoid potential contamination from bovine serum.
The approaches employed can easily be adapted for large-scale materials screening procedures.
The auto-induction process was reliable and convenient for production of cIFN-α protein in E. coli, and can be adapted for large-scale therapeutic protein production.
Furthermore, the use of chick feathers provides an early diagnosis that can be adapted for large-scale analyses being useful for the sex identification of chicks in the first days of nestling life.
Here, we demonstrate that whole genome capture is also a suitable approach for pathogenic bacteria WGS such as A. phagocytophilum, and that it could be adapted for large-scale studies of these bacterial genomes, directly from field samples.
The aim of the present study was to investigate if this protocol could be adapted for large-scale clinical use involving collection of PBMCs by leukapheresis, isolation of monocytes by using the Elutra™ Cell Separation System, and culturing in sterile Teflon bags.
Fractions of bacterially-expressed dsRNA species were prepared using a protocol that was adapted for large-scale purification (12 20 µg/ml of induction) or 96 well microtiter plate purification (V-shape wells, Nunc) [25].
The results clearly demonstrated that the F-MSAP technique can be adapted for use in large-scale DNA methylation detection in the pig genome.
The method used to develop these QIs could be adapted for use in large-scale programmes of hospital regulation in other, including developing, countries.
The entire production seems like it might profitably be adapted for small-scale productions of the piece.
The LIS approach developed here could be employed at a variety of scales in particular for large regions and could be adapted for use as a national scale indicator of habitat availability in heavily disturbed agricultural landscape.
