Sentence examples for be activated by adding from inspiring English sources
In many systems, the pathway can be activated by adding exogenous Wnt protein, or by inhibiting the activity of Gsk3.
Students learn that the transplanted gene can be activated by adding a sugar (arabinose), stimulating the gene to manufacture a protein that looks green under ultraviolet light.
CreERT2 could be activated by adding 200 nM 4-OHT (Sigma, dissolved in absolute ethanol) to the ES cell medium.
Usually, fungal lipases can be activated by adding Mg2+, Ca2+, NH4, and Ba2+ ions to the reaction media [ 45, 51].
After the purified enzyme was activated by adding benzonitrile as substrate for 24 h, the active form of the enzyme with substrate eluted as a peak between ferritin (440 kDa) and thyroglobulin (669 kDa), corresponding to a molecular mass of 496 kDa.
Then, the base liquid of M4M was activated by adding the TBB initiator (3:1 ratio), and a powder/liquid mixture was applied, using a brush-dip technique until the filling of the access hole was completed (Fig. 1).
The excess amount of GA (500 mg, 4.27 mmol) was dissolved in 10 mL PBS solution (pH 7.4), and the carboxylic acid group of GA was activated by adding 10 mL solution of EDC (200 mg, 0.64 mmol) and NHS (200 mg, 1.74 mmol) under stirring conditions and the mixture was kept for 8 h at 5 °C.
DA-PEG block copolymer was synthesized as described previously [6,7].As reported, a solution of 30 mmol dodecanoic acid (DA) and 10 mmol mPEG dissolved in 40 mL of anhydrous dichloromethane was activated by adding 30 mmol of N,N'-dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP).
Before adding to cells, the VacA protein was activated by adding 10% (v/v) of 0.2N HCl [48], then neutralized by reaching a neutral pH.
Briefly, cytosols were activated by adding bovine CC (15 µg/ml) with or without dATP and incubated for 150 min at 37°C.
(i) Elispot plates (Multiscreen HTS, Millipore) were activated by adding ethanol (50 µl, 70%) to each well, then rinsed twice with PBS (160 µl).
