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For both tracers and all time points, images were to be acquired with either a minimum of 800 kcounts per view or an acquisition time of 15 min.
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Images were acquired with either Zeiss AX10 microscope equipped with a SPOT PURSUIT camera or Zeiss LSM 710 confocal microscope.
Fluorescent images were acquired with either a Zeiss LSM-510 VIS or a Perkin Elmer Ultraview VOX spinning disk.
Immunofluorescent images were acquired with either a Nikon A1R microscope (40× objective, numerical aperture 1.30) or with a Zeiss LSM710 microscope (40× objective, numerical aperture 1.30; 63× objective, numerical aperture 1.40).
In most diffusion tensor imaging (DTI) studies, images are acquired with either a partial-Fourier or a parallel partial-Fourier echo-planar imaging (EPI) sequence, in order to shorten the echo time and increase the signal-to-noise ratio (SNR).
Images were acquired with either a Zeiss Axiovert Imager.2 microscope using a 20 × Plan Apochromat (NA 0.8) or 40 × oil Plan Neofluor (NA 1.3) objective and photographed with an AxioCamMRm camera and Zeiss Axiovision software or a Leica SP5 Confocal microscope with LAS software with a 20 × (NA 0.7) objective.
In-gel protein digestion was carried out using modified trypsin, and matrix-assisted laser desorption ionization (MALDI) tandem mass spectrometry (MS/MS) data were acquired with either a 4700 or a 4800 Proteomics Analyzer MALDI tandem time-of-flight (TOF/TOF) mass spectrometer (Applied Biosystems).
Images were acquired with non-descanned detectors.
Images were acquired with 256 × 256 mode.
Images were acquired with a 40× objective.
Images were acquired with H-7650 (Hitachi).
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