Diffusion images will be acquired using single-shot echo planar multi-direction diffusion-weighted sequence, employing dual bipolar diffusion gradient and double spin.
Diffusion tensor imaging (DTI) was acquired using single shot echo-planar imaging, with a 12 channel head coil (TR 12200 ms, TE 94 ms, 72 axial slices, 2 mm thickness, isotropic voxels).
Then the resting state data-sets were acquired using single shot gradient echo EPI (Echo Planar Imaging) with TR 2000 ms and TE 16 ms. Twelve axial slices of 1 mm and a gap of 0.1 mm were recorded with a FOV of (30×30) mm2 and matrix size of 128×128 resulting in voxel dimensions of (0.23×0.23×1) mm3.
Then the resting state data-sets were acquired using single shot gradient echo EPI with TR 2000 ms and TE 15 ms. Twelve axial slices of 0.4 mm and a gap of 0.1 mm were recorded with a FOV of (20×20) mm2 and matrix size of 128×64 resulting in voxel dimensions of (0.16×0.31×0.4) mm3.
On all bio-fluids, standard one-dimensional (1D) H NMR spectra were acquired using single 90° pulse experiment with a total of 64 scans and a relaxation decay of 5 s.
Briefly, BM samples were stained with 2-, 3- and 4-color combinations of moAbs for 15 min in darkness, erythrocytes were lysed with NH4Cl-containing lysing solution for 15 min, washed and data were acquired using single FACS Calibur instrument throughout the study (BD Biosciences, San Jose, CA, USA) flow cytometer.
DTI data was acquired using single-shot spin-echo echo-planar imaging with TR = 9,100, TE = 87 ms, acquisition matrix = 128 × 128 and field of view = 25.6 cm.
BOLD responses were acquired using single-shot, gradient-echo, echo-planar imaging (EPI) with the following parameters: FOV = 192 mm, TR = 2000 ms, TE = 30 ms, flip angle = 90 degrees, and voxel size = 3 × 3 × 3.5 mm.
DTI data were acquired using single-shot spin-echo echo-planar imaging with TR = 10,000 ms, TE = 90 ms, acquisition matrix = 128 × 128 and field of view = 25.6 cm.
Recently we have found that the right MPI can be acquired using a single plane, single waveform technique in fetuses until approximately 26 weeks of gestation when the tricuspid and pulmonary valves diverge (unpublished data).
Delivery input functions (C in(t)) can be measured in a Langendorff perfused heart with a γ-detector coupled to the inflow line, while time-activity curves in the heart (C tiss(t)) can be acquired using a single γ-detector interrogating the entire isolated perfused heart (see Figure 1).
